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You are here:Home » Antibodies » Abs to Calmodulin » Anti -Calmodulin-Dependent Protein Kinase II (CAMKII)

Anti -Calmodulin-Dependent Protein Kinase II (CAMKII)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IF
CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kD (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho- CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Auto-phosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
Catalog #C1035-04F
ApplicationsSuitable for use in ELISA, Immunofluorescence, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml was sufficient for detection of 0.2ug CAMKII by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7L2
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% azide, 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide
SpecificityDetects phosphorylated CaMKII from rat tissues. Specific for alpha and beta subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in beta).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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