| Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions. |
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| Catalog # | C1035-05 |
| Applications | Suitable for use in Western Blot, Immunoprecipation and RIA. Other applications not tested. |
| Recommended Dilutions | Western Blot: 0.1-1ug/ml detects calmodulin (~17kD) in RIPA lysates from human A431 carcinoma cells. Previous lots detected calmodulin in RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C1035-05. Proteins were visualized using a Gt xMo IgG (HRP) and a chemiluminescence detection system. |
| Immunoprecipitation: 4ug immunoprecipitates calmodulin from A431 RIPA cell lysate. |
| RIA: 10ug/ml detects calmodulin in the range of 200-300ng. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-14C: IgG, Fc (HRP) Pab Gt xMo |
| I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo |
| Included Positive Antigen Control | A0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 0.T.172 |
| Host | Mouse |
| Source | Bovine |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to the 21 carboxy-terminal amino acids (aa128-148) of bovine calmodulin, conjugated with tyroglobulin. |
| Specificity | Recognizes both Ca 2+-bound and Ca 2+-free bovine calmodulin. Does not recognize bovine S100- alpha, S100-beta, troponin C or parvalbumin. Species crossreactivity: rat, mouse, human, porcine and wheat germ. Wide crossreactivity expected. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
