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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP IH

Calmodulin (CaM) is a 17 kD protein belonging to a structurally homologous family of proteins which includes troponin C, parvalbumin, and S100. Calmodulin is highly conserved among species and all vertebrate CaMs have identical structures (2). Although CaM exists in abundance in many cells, it can be regulated by hormones and neurotransmitters in a variety of tissues and systems. CaM acts as an intracellular calcium sensor that translates the Ca 2+ signal into a variety of cellular processes. Ca 2+ -CaM recognition of a short polypeptide segment in target proteins induces conformational changes in both CaM and the target, enabling the target protein to become functionally active. Phosphorylation of target proteins at sites on or near the CaM-binding region modulates binding of CaM, thereby providing an additional mechanism of functional regulation.
Catalog #	C1035-05E
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 0.1-0.2ug/ml
	Immunohistochemistry (paraf n-embedded): 2-5ug/ml
	Immunoprecipitation: 2-5ug/IP
	Western Blot: 0.5-1ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3G53
Host	Mouse
Source	Bovine
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide corresponding to the C-terminal region of native bovine brain Calmodulin
Specificity	RRecognizes Calmodulin. (1) All vertebrate Calmodulins are known to have the identical amino acid sequence. No crossreactivity is observed with other calcium binding proteins such as troponin, S-100A, S-100B and parvalbumin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.