| Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+-binding protein acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase. |
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| Catalog # | C1035-09 |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:5000. Detects a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli. Lysates from E. coli transformed with a plasmid encoding a GST-CBP, calmodulin binding protein epitope tagged. Proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with C1035-09. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Concentration | Not Determined |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. |
| Purity | Purified by affinity chromatography. |
| Immunogen | A synthetic peptide, MKRRWKKNFIAVSAANRFKKISSSGAL corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors (KLH). |
| Specificity | Recognizes recombinant proteins containing the calmodulin binding protein epitope tag. Species sequence homology: rabbit, mouse and rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
