Anti -Calmodulin-Dependent Protein Kinase II, pan (CaM Kinase II, CaMKII, CaMK-II, CAMK2,
Calcium/calmodulin-dependent Protein Kinase II alpha, Calcium/calmodulin-dependent Protein
Kinase Type II alpha, CAMKA, CAMK2A Calcium/calmodulin-dependent Protein Kinase
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
|
B |
|
| CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca2+/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1 (4). | | | Catalog # | C1035-25B6 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, | | 0.1% Tween-20 at 4°C with gentle shaking, overnight. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 10E11 | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied in liquid 10mM HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide. | | Immunogen | Synthetic peptide surrounding Val184 of human CaMKII-A (KLH-coupled). | | | Specificity | Recognizes human CaMKII (pan) (D11A10) XPTM. Species crossreactivity: mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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