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Anti -Calmodulin-Dependent Protein Kinase II, phosphorylated (Thr286) (CAMKII)
Anti -Calmodulin-Dependent Protein Kinase II, phosphorylated (Thr286) (CAMKII)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| CaM KII is an important member of calcium/calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling. CaM KII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaM KII further autophosphorylates itself at Thr286 to render the kinase constitutively active. The threonine phosphorylation state of CaM KII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaM KII at Thr286. On the other hand, PKA (protein kinase A) prevents this phosphorylation process through its inhibitory effect on PP1. | | | Catalog # | C1035-25D | | Application(s) | Western Blot. | | Recommended Dilution | Western Blot: 1:1000 (ECL) | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Rat | | Concentration | ~1 mg/ml | | Form | Supplied as a liquid in 10 mM HEPES, pH 7.5, 0.1mg/ml BSA, 50% glycerol. | | Immunogen | Synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated Thr286 | | Specificity | Specific for 50kD and 60kD CaM KII phosphorylated at Thr286. Dot blots and Western blots with phosphopeptide and dephosphopeptide competition was used to define specificity. | | | Important Note | This product is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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