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You are here:Home » Antibodies » Abs to Calmodulin » Anti -Calmodulin-Dependent Protein Kinase II, phosphorylated (Thr286) (CAMKII)

Anti -Calmodulin-Dependent Protein Kinase II, phosphorylated (Thr286) (CAMKII)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
CaM KII is an important member of calcium/calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling. CaM KII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaM KII further autophosphorylates itself at Thr286 to render the kinase constitutively active. The threonine phosphorylation state of CaM KII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaM KII at Thr286. On the other hand, PKA (protein kinase A) prevents this phosphorylation process through its inhibitory effect on PP1.
Catalog #C1035-25D
Application(s) Western Blot.
Recommended DilutionWestern Blot: 1:1000 (ECL)
Clone TypePolyclonal
HostRabbit
SourceRat
Concentration~1 mg/ml
FormSupplied as a liquid in 10 mM HEPES, pH 7.5, 0.1mg/ml BSA, 50% glycerol.
ImmunogenSynthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated Thr286
SpecificitySpecific for 50kD and 60kD CaM KII phosphorylated at Thr286. Dot blots and Western blots with phosphopeptide and dephosphopeptide competition was used to define specificity.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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