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You are here:Home » Antibodies » Abs to Calmodulin » Anti -Calmodulin-Dependent Protein Kinase II (CAMKII)

Anti -Calmodulin-Dependent Protein Kinase II (CAMKII)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
CaMKII is an important member of calcium/ calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates itself at threonine 286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. On the other hand, PKA (protein kinase A) prevents this dephosphorylation process through its inhibitory effect on PP1 (4).
Applications:
Suitable for use in Western Blotting. Other applications not tested.
Catalog #C1035-25E
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100 ug/ml BSA, 50% glycerol.
PurityPurified by protein A and peptide affinity chromatography.
ImmunogenSynthetic peptide (KLH coupled) around the N-terminal amino acid sequence of human CaMKII.
SpecificityDetects endogenous levels of total CaMKII.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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