You are here:Home
»
Antibodies
»
Abs to Calmodulin
»
Anti -Calmodulin-Dependent Protein Kinase II, phosphorylated (Thr286) (CAMKII)
Anti -Calmodulin-Dependent Protein Kinase II, phosphorylated (Thr286) (CAMKII)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| CaMKII is an important member of calcium/ calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates itself at threonine 286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. On the other hand, PKA (protein kinase A) prevents this dephosphorylation process through its inhibitory effect on PP1 (4). | | | Catalog # | C1035-25F | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. | | Purity | Purified by protein A and peptide affinity chromatography. | | Immunogen | Synthetic peptide corresponding to residues around threonine 286 of human CaMKII (KLH). | | Specificity | Recognizes endogenous levels of human CaMKII only when phosphorylated at threonine 286 (Mr 50kD). Species Crossreactivity: mouse, rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
