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You are here:Home » Antibodies » Abs to Calmodulin » Anti -Calmodulin-Dependent Protein Kinase II, Phosphorylated (Thr286) (CaM Kinase II, CaMKII, CaMK-II, CAMK2)

Anti -Calmodulin-Dependent Protein Kinase II, Phosphorylated (Thr286) (CaM Kinase II,
CaMKII, CaMK-II, CAMK2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
CaMKII is an important member of calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1.
Catalog #C1035-25G2
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceRat
ConcentrationNot determined
FormSupplied as a liquid in 10mM HEPES, pH 7.5,150mM sodium chloride,100ug/ml BSA, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Thr286 found in rat brain CaM Kinase II.
SpecificityRecognizes rat CAMK2 when Phosphorylated at Thr286. Species sequence homology: Xenopus, human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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