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You are here:Home » Antibodies » Antibodies-Calmodulin » Anti -Calmodulin-Dependent Protein Kinase II, alpha, beta, phosphorylated (T286/287) (CAMKII)

Anti -Calmodulin-Dependent Protein Kinase II, alpha, beta, phosphorylated (T286/287)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #C1035-25L
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2g/ml detect the phosphorylated alpha and beta subunits of CaM Kinase II in a rat brain microsomal preparation. Pretreating blots with 400U/ml of phosphatase prior to incubation with this lot of antibody abolished the detection of phospho-CaM Kinase II. Treatment with phosphatase had no effect on detection by a
non-phosphospecific antibody to CaM Kinase II.
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide aa281-291 (KMHRQE[pT]VDCLK) corresponding to rat calmodulin- dependent protein kinase II, subunit.
SpecificityRecognizes phospho-CCaM Kinase II, alpha, beta subunits at Mr 50 and 60kD, respectively. Species Crossreactivity
mouse, porcine, Xenopus and Drosophila.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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