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Anti -Calmodulin-Dependent Protein Kinase II, alpha, beta, phosphorylated (T286/287) (CAMKII)
Anti -Calmodulin-Dependent Protein Kinase II, alpha, beta, phosphorylated (T286/287)
(CAMKII)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Catalog # | C1035-25L | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-2g/ml detect the phosphorylated alpha and beta subunits of CaM Kinase II in a rat brain microsomal preparation. Pretreating blots with 400U/ml of phosphatase prior to incubation with this lot of antibody abolished the detection of phospho-CaM Kinase II. Treatment with phosphatase had no effect on detection by a | | non-phosphospecific antibody to CaM Kinase II. | | Optimal dilution determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide aa281-291 (KMHRQE[pT]VDCLK) corresponding to rat calmodulin- dependent protein kinase II, subunit. | | Specificity | Recognizes phospho-CCaM Kinase II, alpha, beta subunits at Mr 50 and 60kD, respectively. Species Crossreactivity | | mouse, porcine, Xenopus and Drosophila. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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