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You are here:Home » Antibodies » Abs to Calnexin » Anti -Calnexin

Anti -Calnexin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH IC IF
Calnexin (CNX), an abundant ~90kD molecular chaperone, is an unglycosylated resident ER transmembrane protein. In mammalian cells, calnexin, together with calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans (4). Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Calnexin associates with numerous oligomeric protein complexes within the ER, including beta1 and alpha6 integrins (5), major histocompatibility class I (6, 7) and class II molecules (11), the antigen receptors expressed on T and B lymphocytes (7), human thyroperoxidase (hTPO) (12), and acetylcholine receptor (13). Recent data also suggest that calnexin might be responsible for the prolonged retention of pro-alpha6 integrin within ER compartment (14). Furthermore, calnexin has been demonstrated to function as a bonafide molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins (15).
Catalog #C1035-26B
ApplicationsSuitable for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry and Electron microscopy. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000 (Colorimetric)
Immunohistochemistry: Paraffin sections
Optimal dilutions to be determined by the researcher.
Positive ControlHeat Shocked HeLa Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceCanine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminus of canine calnexin. The sequence is completely conserved in human, mouse and rat.
SpecificityRecognizes canine Calnexin. MW
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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