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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B FC

Calnexin is calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may be involved in assisting protein assembly or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the ER quality control apparatus via the retention of incorrectly folded proteins.
Catalog #	C1035-26D1
Applications	Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested.
Recommended Dilutions	ELISA: 0.05-0.2ug/ml
	Western Blot: 0.5-1ug/ml
	Flow Cytometry: 1-3ug to label 1x10e6 cells
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide from carboxyl terminal of human calnexin protein, conjugated to KLH (Swiss Prot
Specificity	Recognizes endogenous levels of human calnexin protein. Species Crossreactivity: mouse
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.