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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Calnexin is a calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. As a chaperone protein, it assists in protein assembly by ensuring that only the properly folded protein is progresses along the secretory pathway. It seems to play a major role in the quality control apparatus of the ER by retaining incorrectly folded proteins.
Catalog #	C1035-26D2
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 0.05-0.2ug/ml
	Western Blot: 0.5-1ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.4, 0.02% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide within residues 250-300 of human calnexin protein (Swiss Prot: P27824).
Specificity	Recognizes endogenous levels of human calnexin protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.