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You are here:Home » Antibodies » Abs to Calnexin » Anti -Calnexin - ER Marker

Anti -Calnexin - ER Marker

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH IC
Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.
Catalog #C1035-26G
ApplicationsSuitable for use in Immunocytochemistry/Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunocytochemistry/Immunofluorescence: 1:100
Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:100, using antigen retrieval with sodium citrate buffer (pH 6.0).
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cells, HeLa whole cell lysate, Mouse bladder tissue.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid (serum) in PBS, containing 50% glycerol aand sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenA synthetic peptide corresponding to a portion of the C-terminal between aa550-591 of human Calnexin protein (P35565). Species Sequence Homology: Xenopus, canine, orangutan, rat, bovine, porcine, chicken, Drosophilia, guinea pig, quail, rabbit, sheep 100%.
SpecificityRecognizes human Calnexin at ~90 kD (actual weight
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCANX, CNX, FLJ26570, Histocompatibility complex class I antigen binding protein p88, IP90


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