Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Calpain » Anti -Calpain I (Mu Calpain, Protein Kinase C Activating Factor)

Anti -Calpain I (Mu Calpain, Protein Kinase C Activating Factor)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP IC
Catalog #C1035-27C
ApplicationsSuitable for use in Immunoblot, Immunocytochemistry, Immunoprecipitation and ELISA. Other applications have not been tested.
Recommended DilutionImmunoblot: 1:500. Detects an 80kD large subunit mu-calpain and the subunit in the non-activated proform of the zymogen. Showing a band of ~110kD.
Immunocytochemistry 1:50 on A431 epidermal carcinoma.
ELISA 1:3000 (Against the immunogen).
Immunoprecipitation Calcium is required for large and small subunit association.
Optimal dilutions to be determined by the researcher.
Positive ControlWI-38 human fibroblasts or human erthryocytes
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q42
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, before the addition of 40% glycerol.
PurityAscites
ImmunogenCalpain-I large subunit from human erythrocyte
SpecificityReacts with human calpain-I (mu-Calpain). Does not crossreact with human placental m-calpain (calpain II). This antibody will immunoprecipitate proteolytically active calpains.
Species CrossreactivityHuman, rat, mouse and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links