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Anti -Calpain I (Mu Calpain, Protein Kinase C Activating Factor)
Anti -Calpain I (Mu Calpain, Protein Kinase C Activating Factor)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
E B IP IC |
|
| Catalog # | C1035-27C | | Applications | Suitable for use in Immunoblot, Immunocytochemistry, Immunoprecipitation and ELISA. Other applications have not been tested. | | Recommended Dilution | Immunoblot: 1:500. Detects an 80kD large subunit mu-calpain and the subunit in the non-activated proform of the zymogen. Showing a band of ~110kD. | | Immunocytochemistry | 1:50 on A431 epidermal carcinoma. | | ELISA | 1:3000 (Against the immunogen). | | Immunoprecipitation | Calcium is required for large and small subunit association. | | Optimal dilutions to be determined by the researcher. | | Positive Control | WI-38 human fibroblasts or human erthryocytes | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 2Q42 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid, before the addition of 40% glycerol. | | Purity | Ascites | | Immunogen | Calpain-I large subunit from human erythrocyte | | Specificity | Reacts with human calpain-I (mu-Calpain). Does not crossreact with human placental m-calpain (calpain II). This antibody will immunoprecipitate proteolytically active calpains. | | Species Crossreactivity | Human, rat, mouse and hamster. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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