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You are here:Home » Antibodies » Abs to Calpain » Anti -Calpain I, Domain II (Mu Calpain, Protein Kinase C Activating Factor)

Anti -Calpain I, Domain II (Mu Calpain, Protein Kinase C Activating Factor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IC
Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer
composed of a regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, Calpain 1 ( -calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated posttranscriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain between Gly27 and Leu28 (2). Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development
since limiting its activity could mute invasiveness of tumors or chronic inflammation (1).
Catalog #C1035-27C5
ApplicationsSuitable for use in Immunocytochemistry, Western Blotting. Other applications not tested.
Recommended DilutionImmunocytochemistry: 1:25
Western Blot: 1:1000
Note: Not recommended for Immunoprecipitation.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D196
HostMouse
SourceBovine
FormLiquid. Ascites containing 0.05% sodium azide.
PurityAscites
ImmunogenPurified bovine µ-calpain.
SpecificityRecognizes an epitope in domain II (aa 245-265) of µ-calpain from several mammalian sources (e.g. human, rat, pig, bovine). Detects a band of ~80kDa by Western blot. Does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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