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You are here:Home » Antibodies » Abs to Calpain » Anti -Calpain I, Domain III (Mu Calpain, Protein Kinase C Activating Factor)

Anti -Calpain I, Domain III (Mu Calpain, Protein Kinase C Activating Factor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IC
Catalog #C1035-27C6
Application(s)
Immunocytochemistry (1:100)
Western Blot (1:2000)
Note: Not recommended for immunoprecipitation.
This antibody has been successfully used in Western blot procedures to detect u-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, and rat myoblasts, kidney, liver and spleen. Immunofluorescence staining ofu-calpain in porcine LLC-PK1 cells with 804-050 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D197
HostMouse
SourceBovine
FormLiquid. Ascites containing 0.05% sodium azide.
PurityAscites
ImmunogenPurified bovine µ-calpain.
SpecificityRecognizes an epitope in domain III (aa 465-520) of µ-calpain from several mammalian sources (e.g. human, rat, pig, bovine). Detects a band of ~80kDa by Western blot. Does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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