Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Calpain » Anti -Calpain II, Large Subunit (uCalpain)

Anti -Calpain II, Large Subunit (uCalpain)


  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.


Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IC
Catalog #C1035-27D
ApplicationsSuitable for use in Western Blot, ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:100
ELISA: 1:6,000 (against the immunogen) Immunohistochemistry: 1:100 on 4% paraformaldehyde fixed tissue.
Immunocytochemistry: Tested positive on the following human cells: A431 epidermal carcinoma, MDB-MB-134-VI ductal breast carcinoma, C-33A cervical carcinoma, TE2 esophageal carcinoma, HET-1A esophageal carcinoma and normal human monocytes.
Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a liquid. Contains no preservatives.
PurityPurified by affinity chromatography.
ImmunogenHuman placental m-calpain.
SpecificityRecognizes human m-calpain (Calpain II) large subunit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

External Links