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Anti -Calpain Small Subunit (Mu Calpain)
Anti -Calpain Small Subunit (Mu Calpain)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
E B IP IH IC |
|
| Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Type I Calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium. | | | Catalog # | C1035-27K | | Applications | Suitable for use in ELISA, Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:500-1000. Recognizes the 30kD small subunits of either calpain isozyme. | | ELISA: 1:50,000 | | Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue. | | Immunocytochemistry: 1:100 | | Immunoprecipitation: (small unit, in the presence of calcium large & small complex). | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 2Q30 | | Host | Mouse | | Source | Bovine | | Concentration | As reported | | Form | Supplied as a liquid. No preservative added. | | Purity | Ascites | | Immunogen | Bovine myocardial mu-calpain | | Specificity | Reacts with the small subunit of human erthrocyte u-calpain or bovine myocardial m-calpain. Does not react with calpain large subunits | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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