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You are here:Home » Antibodies » Abs to Calpain » Anti -Calpain Small Subunit (Mu Calpain)

Anti -Calpain Small Subunit (Mu Calpain)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP IH IC
Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Type I Calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.
Catalog #C1035-27K
ApplicationsSuitable for use in ELISA, Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1000. Recognizes the 30kD small subunits of either calpain isozyme.
ELISA: 1:50,000
Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue.
Immunocytochemistry: 1:100
Immunoprecipitation: (small unit, in the presence of calcium large & small complex).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q30
HostMouse
SourceBovine
ConcentrationNot determined
FormSupplied as a liquid. No preservative added.
PurityAscites
ImmunogenBovine myocardial mu-calpain
SpecificityReacts with the small subunit of human erthrocyte u-calpain or bovine myocardial m-calpain. Does not react with calpain large subunits
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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