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You are here:Home » Antibodies » Abs to Calpain » Anti -Calpain (28kD Subunit)

Anti -Calpain (28kD Subunit)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B
The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu- and m-calpains are heterodimers consisting of 28kD and 80kD subunits. The 28kD subunit is identical in the two isoforms, but the 80kD subunits differ with ~50% sequence similarity. 28kD / 80kD complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28kD subunit and results in enzyme activation.
Catalog #C1035-27N
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J487
HostMouse
SourceBovine
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 0.05% sodium azide
PurityAscites
ImmunogenPurified bovine skeletal muscle 28 kDa Calpain subunit.
SpecificityDetects Calpain 28kD subunit from bovine platelet, skeletal and heart muscle and human tissues. This antibody does not crossreact with the human 80kD m-Calpain subunit. Species Crossreactivity: Crossreacts with Human and bovine. Not yet tested in other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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