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You are here:Home » Antibodies » Abs to Calpain » Anti -Calpain 3 (Calpain-3, Calcium-activated Neutral Proteinase 3, CANP 3, CANP3, CAPN3, Calpain L3, CANPL3, Calpain p94, LGMD2, LGMD2A, Muscle-specific Calcium-activated Neutral Protease 3, New Calpain 1, NCL1, nCL-1)

Anti -Calpain 3 (Calpain-3, Calcium-activated Neutral Proteinase 3, CANP 3, CANP3, CAPN3,
Calpain L3, CANPL3, Calpain p94, LGMD2, LGMD2A, Muscle-specific Calcium-activated Neutral
Protease 3, New Calpain 1, NCL1, nCL-1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B
Calpain 3 is a muscle-specific calcium-activated neutral protease. Defects in the gene encoding for calpain 3 appear to be the origin of a form of limb-girdle muscular dytrophy. This antibody will help detect the presence or absence of calpain 3 in western blotting and maybe useful in further characterizing calpain 3 expression.
Catalog #C1035-27V
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:25-1:50
Optimal dilutions to be determined by the researcher.
Positive Control
Freshly isolated normal human skeletal muscle homogenate prepared in treatment buffer containing 0.125M tris-HCl buffer pH6.4, 10% glycerol, 4% SDS, 4M urea, 10% mercaptoethanol and 0.001% bromophenol blue (final pH of treat- ment buffer, 6.8). Bands at approx- imately 94kD and 30kD.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone NoCalp3d/2C4
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 2.5ml dH2O.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to aa1-19 of human calpain 3.
SpecificityRecognizes human Calpain 3. Species Crossreactivity: Canine and rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCalpain 3, (p94), EC=3.4.22.54


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