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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -Calponin (Basic Calponin, Calponin 1, Calponin 1 Basic Smooth Muscle, Calponin H1 Smooth Muscle, Calponin1, Calponins Basic, CNN 1, CNN1, Sm Calp, SMCC)

Anti -Calponin (Basic Calponin, Calponin 1, Calponin 1 Basic Smooth Muscle, Calponin H1
Smooth Muscle, Calponin1, Calponins Basic, CNN 1, CNN1, Sm Calp, SMCC)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Calponin, a calmodulin, binds tropomyosin and F-actin and is thought to be involved in the regulation of smooth muscle contraction. The expression of calponin is restricted to smooth muscle cells and is a marker of the differentiated contractile phenotype of developing smooth muscle. Two isoforms of calponin exist whose molecular weights are 34kD and 29kD. Expression of the 29kD form is primarily restricted to muscle of the urogenital tract. The expression of calponin has also been demonstrated in myoepithelial cells from benign and malignant breast lesions.
Catalog #C1035-40
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlMyoepithelial cells in breast ducts.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.39
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenCrude human uterus extract. Cellular Localization
SpecificityRecognizes the 34kD isoform of human Calponin. Species Crossreactivity: Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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