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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Calprotectin (S100A8/A9, MRP-8/MRP-14)

Anti -Calprotectin (S100A8/A9, MRP-8/MRP-14)

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Purified E IH
This polyclonal antibody recognizes different epitopes on the MRP8/14 heterocomplex (S100A8/S100A9). It is particularly suited for use in ELISA or similar assays. It was generated by immunising chickens with recombinant MRP8/14. Thus, the antibody does not discriminate between the individual subunits MRP8 or MRP14. In immune histochemistry, it detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears. The MRP8/14 specific ELISA has shown significant advantages in the early assessment of certain acute inflammatory conditions.
Catalog #C1035-61G
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Immunohistochemistry (Frozen): 1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgY
HostChicken
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenRecombinant corresponding to human MRP8/14.
SpecificityRecognizes human MRP8 and MRP14 and their Ca2+-dependent complex. Several epitopes. Species Crossreactivity: bovine and porcine (spleen).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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