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You are here:Home » Antibodies » Abs to Binding Proteins, Calcium » Anti -Calreticulin (CALR, SSA, Sicca Syndrome Antigen A, Ro, Autoantigen Ro)

Anti -Calreticulin (CALR, SSA, Sicca Syndrome Antigen A, Ro, Autoantigen Ro)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Calreticulin was originally identified as a Ca2+ binding protein in the muscle sarcoplasmic reticulum. Since its original discovery, it has been found in high abundance in non-muscle tissue. Calreticulin has the ability to bind Ca2+ with high capacity but low affinity. The exact function of calreticulin is not yet known, however recent reports suggest that it may play a role in nuclear hormone receptor gene transcription and may also act as a chaperone protein. Calreticulin is 90% conserved among human, mouse, rat, rabbit and Aplysia.
Catalog #C1036
ApplicationsSuitable for Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects calreticulin in RIPA lysates from murine 3T3/A31 cells. 3T3/A31 cell lysates were resolved by electrophoresis, transferred to PVDF and probed with anti-human Calreticulin (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunohistochemistry: Detects calreticulin in tissue sections and cells.
Optimal diltions to be determined by the researcher.
Included Positive Antigen ControlF4209: Fibroblast Cell, 3T3/A31. Use 20ug per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a frozen solution in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin. Conjugated to KLH.
SpecificitySpecific for calreticulin. Does not crossreact with other endoplasmic reticulum proteins. Species Crossreacitivity: Human, mouse, rat, Xenopus and weakly with bovine and porcine. No reaction with chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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