| Calreticulin was originally identified as a Ca2+ binding protein in the muscle sarcoplasmic reticulum. Since its original discovery, it has been found in high abundance in non-muscle tissue. Calreticulin has the ability to bind Ca2+ with high capacity but low affinity. The exact function of calreticulin is not yet known, however recent reports suggest that it may play a role in nuclear hormone receptor gene transcription and may also act as a chaperone protein. Calreticulin is 90% conserved among human, mouse, rat, rabbit and Aplysia. |
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| Catalog # | C1036 |
| Applications | Suitable for Immunoblot and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilution | Immunoblot: 0.5-2ug/ml detects calreticulin in RIPA lysates from murine 3T3/NIH fibroblasts and human foreskin fibroblast (HFF). 20ug 3T3/NIH and 10ug HFF cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-human Calreticulin (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Immunohistochemistry: Detects calreticulin in tissue sections and cells. |
| Optimal diltions to be determined by the researcher. |
| Included Positive Antigen Control | 3T3 lysate. Use 20ug per lane for minigels. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a frozen solution in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin, KLH conjugated. |
| Specificity | Specific for calreticulin. Does not crossreact with other endoplasmic reticulum proteins. Species Crossreacitivity: Human, mouse, rat, Xenopus and weakly with bovine and porcine. No reaction with chicken. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
