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You are here:Home » Antibodies » Abs to Calretinin » Anti -Calretinin (Calbindin, CAB29, CAL2)

Anti -Calretinin (Calbindin, CAB29, CAL2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural motif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.
Catalog #C1036-01
ApplicationsSuitable for use in Immunohistochemistry. Not recommended for Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Formalin/paraffin): 1:40-1:80 for 60 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlMesothelioma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.40
HostMouse
SourceMouse
ConcentrationNot Determined
FormSupplied as a liquid, 0.09% sodium azide.
PurityTissue culture supernatant
ImmunogenRecombinant, full length mouse calretinin protein. MW of Antigen
SpecificityRecognizes mouse Calretinin. Species Crossreactivity: human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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