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You are here:Home » Antibodies » Abs to Calretinin » Anti -Calretinin (Calbindin, CAB29, CAL2)

Anti -Calretinin (Calbindin, CAB29, CAL2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E IH
Calretinin is an intracellular calciumbinding protein belonging to the troponin C superfamily characterized by a structural motif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.
Catalog #C1036-01H
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:10-1:20 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlMesothelioma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5G28
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, 0.09% sodium azide.
PuritySupernatant
ImmunogenRecombinant full length human calretinin protein cloned from Mero-41 cells. Cellular Localization: Cytoplasmic
SpecificityRecognizes human Calretinin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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