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You are here:Home » Antibodies » Abs to Calretinin » Anti -Calretinin (Calbindin, CAB29, CAL2)

Anti -Calretinin (Calbindin, CAB29, CAL2)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant E IH
Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural motif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.
Catalog #C1036-01J
ApplicationsImmunohistology (Formalin/paraffin): (Use Ab 1:100-200 for 30 min) Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Excellent for staining of formalin/paraffin.
Positive Control Mesothelioma
Cellular Localization Cytoplasmic and nuclear
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No5G26
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid with 0.09% sodium azide.
PurityCulture supernatant
ImmunogenRecombinant full-length mouse calretinin protein.
SpecificityRecognizes Calretinin. Species Crossreactivity: Human, Mouse, and Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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