Calbindin D29K
Calretinin, also known as Calbindin 2, belongs to the calbindin family of proteins. It has six EF-hand domains which bind calcium.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.1-0.5ug/ml Immunohistochemistry (FFPE): 0.5-2ug/ml. Antigen retrieval using heat is required. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa4-18, PQQQPPYLHLAELTA from the N-terminus of human Calretinin
Form
Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
Purity
Purified by affinity chromatography
Specificity
Recognizes human Calretinin at 32kD. Species Crossreactivity: mouse, rat
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