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You are here:Home » Antibodies » Abs to Calretinin » Anti -Calretinin (CR, 29kD Calbindin, CAB29, CAL2, CALB2, Calbindin 2 29kD, Calbindin-2, Calbindin D29K)

Anti -Calretinin (CR, 29kD Calbindin, CAB29, CAL2, CALB2, Calbindin 2 29kD, Calbindin-2,
Calbindin D29K)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural mitif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.
Catalog #C1036-01K1
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 2.5-5ug/ml
Immunohistochemistry: 1:200 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissues sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlBrain and mesothelioma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to full-length mouse calretinin.
SpecificityRecognizes mouse calretinin. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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