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Abs to Calretinin
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Anti -Calretinin (CR, 29kD Calbindin, CAB29, CAL2, CALB2, Calbindin 2 29kD, Calbindin-2, Calbindin D29K)
Anti -Calretinin (CR, 29kD Calbindin, CAB29, CAL2, CALB2, Calbindin 2 29kD, Calbindin-2,
Calbindin D29K)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH |
|
| Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural mitif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas. | | | Catalog # | C1036-01K1 | | Applications | Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 2.5-5ug/ml | | Immunohistochemistry: 1:200 for 10min at RT | | Staining of formalin-fixed tissues requires boiling tissues sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min | | Optimal dilutions to be determined by the researcher. | | Positive Control | Brain and mesothelioma | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Mouse | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to full-length mouse calretinin. | | Specificity | Recognizes mouse calretinin. Species Crossreactivity: human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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