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You are here:Home » Antibodies » Abs to Binding Proteins, Calcium » Anti -Calreticulin (CALR, SSA, Sicca Syndrome Antigen A, Ro, Autoantigen Ro)

Anti -Calreticulin (CALR, SSA, Sicca Syndrome Antigen A, Ro, Autoantigen Ro)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP IH IC IF
Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER). It is also a molecular chaperone and is involved in protein folding events in the ER. Calreticulin has also been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (2-3). Recently it has been shown that calreticulin is induced in response to various types of cell stress including amino acid deprivation (1).
Catalog #C1036-02A
Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and crucially participate in the coordination of these functions by the cell (2-3).
ApplicationsSuitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1,000 (ECL). A band is detected at ~55kD.
Immunocytochemistry: 1:250
Immunohistochemistry: 1:50
Immunofluorescence: 1:50
Immunoprecipitation: 1:300
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
PuritySerum
ImmunogenFusion protein to mouse calreticulin
Cellular LocalizationCalreticulin staining is localized to the ER in most cell types and under most conditions (1,2).
SpecificityRecognizes mouse calreticulin. Species Crossreactivity: Human, bovine, rat and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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