Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Binding Proteins, Calcium » Anti -Calreticulin (CALR, SSA, Sicca Syndrome Antigen A, Ro, Autoantigen Ro)

Anti -Calreticulin (CALR, SSA, Sicca Syndrome Antigen A, Ro, Autoantigen Ro)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Calreticulin (CRT) is a multifunctional, multi-compartmental protein most abundant in the ER lumen, but also found in other membrane-bound organelles, the cell surface and extracellularly. Reports of nuclear localization are likely immunostaining artifacts (7). CRT is also known as CRP-55, calregulin and HACBP (high affinity calcium-binding protein) (7,8). It contains the ER-retrieval sequence, KDEL, and has been best characterized as a soluble molecular chaperone of new or misfolded proteins and a Ca2+-binding protein. CRT is comprised of three domains; a 180 residue N-terminal domain, a proline-rich P-domain (residues 189288) that binds Ca2+ with high affininty and has homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (7,9). The P-domain may interact with the cochaperone ERp57 (Grp58), a thiol reductase. The NMR structure of the P-domain (residues 189288) is an extended hairpin that seems to form an extended, somewhat curved protrusion from the CRT core domain (9). Both CRT and its membrane bound homolog, CNX, interact with proteins and glycoproteins that have monoglucosylated N-glycans (7,9,10). The CRT/CNX cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligimerization, regulates ER degradation and provides quality control by preventing incompletely folded glycoproteins from exiting to the Golgi complex (9,10). Other functions atttributed to CRT include adhesion, blood function, cardiac and neuronal development gene expression and other diverse functions. CRT is also implicated as an autoantigen in systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block and halothane hepatitis (7).
Catalog #C1036-02G
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Positive ControlsHeLa Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 0.09% azide, 50% glycerol
PurityPurified by Protein A affinity chromatography
ImmunogenSynthetic 13aa peptide sequence (aa405-417) from human calreticulin with a cysteine residue added (KLH coupled).
SpecificityRecognizes human Calreticulin at ~63kD. Antibody can immunoprecipitate native Calreticulin. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links