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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP IC IF

Calsequestrin is a major Ca2+ binding protein of the sarcoplasmic reticulum (SR) that is found in the terminal cisternae of the SR. It is an acidic protein with a large capacity (40–50 moles of Ca2+ per mole of protein) but moderate to low affinity (1mM) for Ca2+ (1,2). Calsequestrin apparently buffers free Ca2+ in the cisternae of the SR and thereby reduces the gradient against which the Ca2+-ATPase must pump Ca2+. It also localizes Ca2+ at the junctional face of terminal cisternae for rapid release into the cytoplasm via the ryanodine receptor (3). Mammalian isoforms of calsequestrin specific for cardiac and skeletal muscle have been identified (4,5).
Catalog #	C1036-05
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilution	Western Blot: 0.5ug/ml detected the 60kD calsequestrin in sarcoplasmic reticulum preparation from rat heart ventricles.
	Immunoprecipitation: 5ug/ml immunoprecipitated calsequestrin from a lysate of sarcoplasmic reticulum preparation from rat heart ventricles.
	Immunocytochemistry: 10ug/ml detected calsequestrin in frozen sections of rat heart by immunofluorescence.
	Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39	IgG, X-Adsorbed (HRP) Pab Gt xRb
	I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
	I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Canine
Concentration	~1mg/ml
Form	Supplied as a liquid in 0.1M Tris-glycine buffer, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Ten residue synthetic peptide (ESVSSDKVAQ) corresponding to amino acids 39-48 of mature canine cardiac calsequestrin.
Specificity	Recognizes canine calsequestrin, cardiac. Species Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.