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C1036-36 Sheep Anti-Calsyntenin-2 (Calsyntenin 2, Alcadein-gamma, Alc-gamma, CLSTN2, CS2)

Specifications
Clone Type
Polyclonal
Host
Sheep
Source
Human
Swiss Prot
Q9H4D0
Isotype
IgG
Grade
Affinity Purified
Applications
IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Calsyntenin-2 (CS-2) is a 120-135kD member of the Alcadein family of molecules. It is expressed in the ER/Golgi, and the plasma membranes of almost all neurons. It is a calcium-binding protein that interacts with APP and X11L. In this regard, it appears to regulate APP cleavage and gene activation, and likely impacts post-synaptic signaling. Notably, when cleaved in a manner similar to APP, its intracellular fragment antagonizes AICD gene activation. Mature human CS-2 is a 935aa type I transmembrane protein. It contains two cadherin domains aa44-280 in its extracellular region aa21-831 and a 103aa cytoplasmic tail. Proteolytic cleavage by ADAM10 occurs between His803 and Leu804 to generate a 105-110kD extracellular fragment. Additional processing of the transmembrane fragment by g-secretase creates a 3kD fragment aa804-834 plus a 25kD cytoplasmic protein. Potential isoform variants show a premature truncation after Gln608, and the use of an alternative start site at Met409. Over aa21-834, human CS-2 shares 95aa identity with mouse CST-2.

Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ser21-Thr834 (Val366Ile) from human Calsyntenin-2 expressed in NSO cells.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Calsyntenin-2. Species sequence homology: human Calsyntenin-1 and Calsyntenin-3 (1%).
Conjugates
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