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You are here:Home » Molecular Biology » MB-Protease Inhibitors » Calyculin A (Serine/Threonine Phosphatase Inhibitor)

Calyculin A (Serine/Threonine Phosphatase Inhibitor)


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Calyculin A inhibits the activity of protein phosphatases PP1 and PP2A (1,2). Unlike Okadaic acid, which reduces PP2A activity but has little effect on PP1 activity, calyculin A inhibits both phosphatases (1). Neither calyculin A nor Okadaic acid inhibit acid or alkaline phosphatases or phosphotyrosine protein phosphatases (2). Calyculin A (Serine/Threonine Phosphatase Inhibitor) is a much more potent phosphatase inhibitor than Okadaic acid (2). As shown in the first Western blot, treating cells with 100nM Calyculin A for 30 minutes can dramatically induce threonine phosphorylation as detected by this antibody. IC50 values for inhibitory activity against PP1 are around 2nM. IC50 values for inhibitory activity against PP2A are around 0.5-1nM.
Catalog #C1036-72
ApplicationsSuitable for use as an inhibitor of protein phosphatases PP1 and PP2A. Other applications not tested
Recommended DilutionTreat cells with 50-100nM calyculin A for 5-30 minutes.
Optimal dilutions to be determined by the researcher
Storage and StabilityReconstitute lyophilized clear film. with 1ml DMSO, aliquot, tightly seal and store at -20° C in the dark. Reconstituted product is stable for 6 months at -20°C.
Molecular Weight1009.18
FormSupplied as a lyophilized clear film. For 10uM stock, reconstitute in 1ml DMSO.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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