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You are here:Home » Antibodies » Abs to Calmodulin » Anti -CaM Kinase II, alpha (Calmodulin-Dependent Protein Kinase II)

Anti -CaM Kinase II, alpha (Calmodulin-Dependent Protein Kinase II)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Catalog #C1036-76
Applications Suitable for use in Immunoblot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilution Immunoblot Analysis: 0.52ug/ml detects CaM Kinase II, alpha subunit, in bovine brain cytosol and rat brain microsome preparations. Bovine brain cytosol was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CaM Kinase II (0.5ug/ml). Proteins were visualized using a goat antimouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunohistochemistry: This antibody has been reported to detect CaM Kinase II, alpha subunit, in rat brain coronal sections.1
Storage and Stability May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4i28
HostMouse
SourceRat
Concentration~1mg/ml
Form100ug of mouse IgG1 in 100ul of 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% azide containing 30% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenPartially puri ed rat CaM Kinase II, a-subunit.
SpecificityRecognizes the a subunit of CaM Kinase II, Mr 50kDa, with high af nity and speci city.1 Species Crossreactivity: Rat and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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