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You are here:Home » Antibodies » Abs to Calmodulin » Anti -CaM Kinase II, alpha, phosphorylated (Thr286) (Calmodulin-Dependent Protein Kinase II)

Anti -CaM Kinase II, alpha, phosphorylated (Thr286) (Calmodulin-Dependent Protein Kinase
II)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Catalog #C1036-77
ApplicationsSuitable for use in ELISA and Immunoblot. Other applications not tested.
Recommended DilutionsImmunoblot: 0.5-2.0ug/ml
Optimal dilutions to be determined by the researcher.
Immunoblot Analysis2ug/ml detected phosphorylated CaM kinase II a subunit, Mr ~50kD, in a rat brain microsomal protein preparation. Pretreating blots with lambda phosphatase (400 units/ml), prior to incubation reduced the signal strength. Treatment with lambda phosphatase had no effect on the signal strength using a non-phosphospeci c CaM Kinase II antibody. A rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and incubated without lambda phosphatase or incubated with lambda phosphatase before being probed with anti-phospho-CaM Kinase II (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3i29
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 281294 (MHRQE[pT]-VDCLKKFN) of rat Cam Kinase II a-subunit.
SpecificityRecognizes phospho-CaM Kinase II a subunit, Mr 50kD. Additional proteins observed at 125kD and 135kD.
Species crossreactivityRat. Predicted to react with mouse, human and chicken based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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