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You are here:Home » Antibodies » Abs to Calmodulin » Anti -CAMKIV (Calcium/Calmodulin-dependent Protein Kinase Type IV, CAM Kinase-GR, CaMK IV, CAMK4)

Anti -CAMKIV (Calcium/Calmodulin-dependent Protein Kinase Type IV, CAM Kinase-GR, CaMK IV,
CAMK4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Calmodulin dependent protein kinases, with a molecular weight of 65kD, consist of a family of Ser/Thr protein kinases including CaMK I, CaMK II, and CaMK IV. CaMKs are considered to play a central role in many cellular functions in response to Ca2+ signaling, including synthesis and secretion of neurotransmitters, axonal transport, long term potentiation, etc. CaM kinase IV is abundantly expressed in the brain and the thymus, and is localized o the cytoplasm and nucleus.
Catalog #C1036-85M
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2.5-5ug/ml
Immunohistochemistry: 1:50 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at room temperature for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat cells. Brain
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceRat
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa457-474 of rat CaM kinase IV.
SpecificityRecognizes rat CaM kinase IV. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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