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You are here:Home » Kits and Assays » Kits for ELISA (Food Safety), BioAssay™ » Campylobacter, BioAssay™ ELISA Kit

Campylobacter, BioAssay™ ELISA Kit

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Specifications

Infection by thermophilic Campylobacter species is a leading cause of human gastroenteritis. Of the various species of Campylobacter, C. jejuni, C. coli and C. lari, are the species most often associated with human illness. Campylobacter are often passed to humans through the handling or consumption of contaminated food, particularly foods of animal origin.
Catalog #C1037-19
Recently, human infection with Campylobacter has been implicated in the induction of Guillain- Barré Syndrome (GBS) and reactive arthritis. GBS is a debilitating and potentially fatal neurological disease that produces paralysis.
Campylobacter species are gram negative, motile curved or spiral rods that require highly specialized growth conditions. Typical cultivation entails pre-enrichment and enrichment steps in broth, followed by isolation on a selective solid medium. Of particular importance in the cultivation of Campylobacter is the requirement for a microaerobic atmosphere.
Cultivation of thermophilic Campylobacter species from foods involves multiple media steps, and thus many days. The United States Biological Campylobacter Microwell ELISA allows rapid testing (less than an hour) of the enrichment broth, thus eliminating the need for the plating and identification steps used in the standard methods. The United States Biological Campylobacter Microwell ELISA reduces time to result by several days.
Intended UseThe United States Biological Campylobacter assay is an enzyme-linked immunosorbent assay (ELISA) that may be used to screen food products for the presence of thermophilic Campylobacter antigen.
Test PrincipleThe United States Biological Campylobacter ELISA is a double antibody (sandwich) ELISA utilizing specific anti-Campylobacter antibodies coated to microwells. After addition of the sample and the enzyme conjugate, a positive reaction (indicating the presence of Campylobacter antigen) produces a deep blue color. Addition of the Stop Solution ends the assay and turns the blue color to yellow. The results may be read visually or with an ELISA reader.
Kit Components1. Microtiter plate 1x96 wells each.
2. Enzyme Conjugate: 1x11ml of peroxidase conjugated anti-Campylobacter polyclonal antibody with red dye and a preservative.
3. Positive control: 1 vial x 2ml of Campylobacter antigen in a buffered base.
4. Negative control: 1 vial x 2ml of buffered base.
5. Chromogen: 1x11ml of chromogen tetramethylbenzidine (TMB).
6. Wash Concentrate (20X): 1x25 ml of concentrated buffer and surfactant with preservative.
7. Stop solution: 1x11ml of 1M phosphoric acid.
Storage and StabilityStore components at 4°C. Stable for at least 6 months .For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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