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You are here:Home » Antibodies » Abs to Lectins » Anti -Canavalia ensiformis (Concanavalin A, Jackbean)

Anti -Canavalia ensiformis (Concanavalin A, Jackbean)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified
Isolated from Canavalia ensiformis (Jack bean) seeds Con A is one of the most widely used and well characterized lectins. Con A has broad applicability primarily because it recognizes a commonly occurring sugar structure, a-linked mannose. Since a wide variety of serum and membrane glycoproteins have a “core oligosaccharide” structure which includes a-linked mannose residues, many glycoproteins can be examined or purified with Con A and its conjugates. Con A has been utilized in hormone receptor studies, mitogenic assays, characterization of normal and malignant cells, glycoprotein purification, viral antigen isolation, dextran and mannan fractionation, cell agglutination studies, bacterial aggregation, membrane fluidity and lateral mobility investigations, turbidimetric assays for sugars, lymphokine production, as well as in many other applications. At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits of approximately 26kD each. Below pH 5.6, Con A dissociates into active dimers of 52kD Acetylation, succinylation or other derivatizations can also produce stable forms with dimeric structures. “Native” Con A is a mixture of several forms of the lectin due to “nicks” occurring in the polypeptide chains. Although having little or no effect on the saccharide binding activity, these “nicks” in the sequence are often revealed even in the purest lectin preparations as additional bands in SDS-polyacrylamide gel electrophoresis. These hydrolytic cleavage sites appear to exist in the lectin as it occurs in the seeds and are not a function of isolation procedures.
Catalog #C1045-04
ApplicationsSuitable for use in Immunodiffusion and IEP. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
PurityPurified by ammonium sulfate precipitation.
SpecificityRecognizes Canavalia ensiformis (Concanavalin A).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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