| Con A is a tetramer of four identical ~26kD subunits at or above neutral pH. Below pH 5.6, Con A dissociates into active dimers of ~52,000D. Con A recognizes alpha-linked mannose found in many glycoproteins. |
|
| Catalog # | C1045-10 |
| Lectins are derived from the extracts of plants, animals, viruses and microorganisms. They are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. |
| Lectins form precipitates with glycoconjugates and are which is useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. |
| Canavalia ensiformis-agarose conjugate is prepared from affinity-purified lectin and 4% cross-linked agarose beads. The Molecular weight exclusion limit is ~2x10e7. Binding capacity per mg of lectin | is reported on each Certificate of Analysis. |
| Notes on Use | • Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. |
| • 0.2M alpha-methyl mannoside/0.2M alpha-methyl glucoside mixture is recommended for elution of glycoconjugates which bind to immobilized lectin |
| CAS Number | n/a |
| Molecular Weight | 102kD |
| Source | Jack Bean (Canavalia ensiformis) |
|
| Purity | Canavalia ensiformis-agarose conjugate is prepared from affinity purified lectin and 4% ross-linked to agarose beads. |
|
| Quality Control | SDS-Page |
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| Isoelectric Point | ~pH 5 |
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| Binding Site Requirements | Ca++, Mn++ |
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| Carbohydrate Specificity | |
| a-mannose, a-glucose, branched mannose |
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| Inhibitory Carbohydrates | |
| Methyl-a-D-mannopyranoside --> a-D-mannose --> a-D-glucose |
|
| Activity | |
| Con A is a relatively weak blood agglutinin. Suggested concentration of 10ug/ml required to yield visible agglutination of neuraminidase-treated human erythrocytes. |
|
| Molecular weight exclusion limit | ~2x10e7 |
|
| Binding Capacity | > 4mg/ml ovalbumin/ml gel |
|
| Protein Concentration | ~6mg/ml of settled gel |
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| Form | Supplied as a suspension in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.1mM calcium chloride, 0.01mM manganese chloride, 20mM glucose, 0.08% sodium azide. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | Jack bean |
