Anti -Capsaicin Receptor (VR1, Transient receptor potential cation channel subfamily V
member 1, TrpV1, osm-9-like TRP channel 1, OTRPC1, Vanilloid receptor 1, Vanilloid
receptor type 1-like)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B IH IC IF |
|
| Catalog # | C1078-25 | | Applications | Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Indirect Immunofluorescence. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:1000 | | Immunohistochemistry: 1:1000. Used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at RT. C1078-25 was diluted. Blocking buffer was removed and slides were incubated for 18-24 hours at 4°C with C1078-25. Slides were rinsed 3x and then incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3x and coverslipped. Staining was examined using fluorescence microscopy. | | Immunocytochemistry: 1:1000. VR1 transfected cells were processed for indirect Immunofluorescence. Media was removed and cells were gently washed 3x with serum-free media. Following fixation, cells were processed for indirect Immunofluorescence as described above. | | Note: sodium azide interferes with peroxidase reactions and should not be used with peroxidase methodologies. If sodium azide is present in any steps of the stainig procedure, the tissue should be thouroughly washed with sodium-free buffer before performing the peroxidase reaction. | | Optimal dilutions to be determined by the researcher. | | Control peptide | C1078-25A: Capsaicin Receptor, NT, Control Peptide (Vanilloid Receptor 1, VR1) | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | Not Determined | | Form | Supplied as a liquid, 0.05% sodium azide. | | Purity | Serum | | Immunogen | Synthetic peptide corresponding to residues 4-21, RASLDSEESESPPQENSC, of the amino-terminus of rat Capsaicin Receptor. | | Specificity | Recognizes rat Capsaicin Receptor, NT (Vanilloid Receptor 1, VR1). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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