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You are here:Home » Antibodies » Abs to Ion Channel » Anti -Capsaicin Receptor (VR1, Transient receptor potential cation channel subfamily V member 1, TrpV1, osm-9-like TRP channel 1, OTRPC1, Vanilloid receptor 1, Vanilloid receptor type 1-like)

Anti -Capsaicin Receptor (VR1, Transient receptor potential cation channel subfamily V
member 1, TrpV1, osm-9-like TRP channel 1, OTRPC1, Vanilloid receptor 1, Vanilloid
receptor type 1-like)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Capsaicin receptor, termed vanilloid receptor subtype 1 (VR1), has been cloned from sensory neurons. VR1 protein is a heat-gated cation channel. VR1 is also activated by noxious heat (elevation in temperature causing pain) and high proton concentrations (pH <6.0). Rat VR1 encodes a membrane protein of 838aa (~95kD) with 6 transmembrane domains. VR1 expression is limited to small to medium diameter primary sensory neurons. Recently, a structural homolog of VR1, termed VRL-1 (VR1 like proteins; rat 761aa, human 764aa) have been cloned and characterized. There is ~49% identity between VRL-1 and VR1. In contrast to VR1, VRL-1 does not respond to capsaicin, acid or moderate heat. Instead, it is activated by high temperatures with a threshold of ~52°C. Within sensory ganglia, VRL-1 is most prominently expressed by a subset of medium to large-diameter neurons, and in many non-neuronal tissues (lung, spleen and intestine) that are unlikely to encounter temperatures of >50°C. It is possible that VRL-1 is also activated by stimuli other than heat.
Catalog #C1078-25B
ApplicationsSuitable for use in Western Blot and ELISA Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000. Recombinant VR1 is ~90kD
ELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control Peptide V2075-05A
Reconstitution
Reconstitute with 100ul of sterile dH2O.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and store at -20°C. Do not freeze. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid, serum, 0.1% sodium azide.
PuritySerum
ImmunogenSynthetic peptide (18aa) corresponding to the C-terminus of the rat VRL-1 protein.
SpecificityRecognizes Vanilloid receptor like protein 1 (VRL-1). No significant sequence homology is found with VR1 or other proteins. Species crossreactivity: 100% conserved in mouse VRL-1 and growth factor regulated calcium channel. Only 52% homology with human VRL-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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