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You are here:Home » Antibodies » Abs to Enzymes » Anti -Carbonic Anhydrase IX (CA9)

Anti -Carbonic Anhydrase IX (CA9)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IF
Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.
Catalog #C1105-80E
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Immunofluorescence: 1:1000
Western Blot: 1:2000
Immunohistochemistry (Frozen): 1:1000. Can also be used with paraffin sections.
Optimal dilutions to be determined by the researcher.
Positive ControlRenal carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-citrate/phophate, pH 7.5, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to a C-terminal sequence of the human carbonic anhydrase IX. Cellular Localization: Cell membranes
SpecificityRecognizes human carbonic anhydrase IX. Species Crossreactivity: canine, mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCA9, CAIX, Carbonic dehydratase, Membrane antigen MN, P54/58N, pMW1, RCC associated protein G250, Renal cell carcinoma associated antigen G250, CA IX


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