| Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia. |
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| Catalog # | C1105-80E |
| Applications | Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. |
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| Recommended Dilutions: |
| Immunofluorescence: 1:1000 |
| Western Blot: 1:2000 |
| Immunohistochemistry (Frozen): 1:1000. Can also be used with paraffin sections. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Renal carcinoma |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in Tris-citrate/phophate, pH 7.5, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to a C-terminal sequence of the human carbonic anhydrase IX. Cellular Localization: Cell Membranes |
| Specificity | Recognizes human carbonic anhydrase IX. Species Crossreactivity: canine and rat |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | CA9, CAIX, Carbonic dehydratase, Membrane antigen MN, P54/58N, pMW1, RCC associated protein G250, Renal cell carcinoma associated antigen G250, CA IX |
