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You are here:Home » Antibodies » Abs to Enzymes, Carboxypeptidase » Anti -Carboxypeptidase A2

Anti -Carboxypeptidase A2

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP N
Carboxypeptidase A2 (CPA2) cleaves C-terminal residues with aromatic side chains
including Trp, Tyr and Phe. The 411 aa rhCPA2 consists of a pro region (aa 17 to 112) and
a mature chain (aa 113 to 417).
Catalog #C1204-07
ApplicationsSuitable for use in Western blot, Neutralization, Direct ELISA and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions:
Western blot:1-2ug/ml the detection limit is~10 ng/lane under non-reducing and reducing conditions. will increase sensitivity by 5 to 50 fold.
Neutralization: preincubate with the activated enzyme at different molar ratios at room temperature for 30 min. Then assay with N-acetyl-Phe-Thiaphe-OH. 50% of the enzymatic activity was inhibited at~ 1.5 ug/ml (IC50) under conditions in which the enzyme was
present at 0.1 ug/ml and the substrate concentration was 0.1 mM. Considering the
molecular masses of the enzyme (42kD) and the antibody (150kD), IC50 was achieved at
approximately 5:1 molar ratio of the antibody to the enzyme.
Immunoprecipitation: 25 ug/ml
Direct ELISA: 0.5-1.0 ug/mL
Optimal dilutions to be determined bythe researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No8B4
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS with 5% trehalose . Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived recombinant human rhCPA2.
SpecificityRecognizes the pro form of human CPA2, but not the activated enzyme in Western blots. Species sequence homology: chimpanzee 99.5%, canine 90%, rat 87%, mouse 86% and chicken 70%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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