| Carboxypeptidase A2 (CPA2) cleaves C-terminal residues with aromatic side chains |
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| including Trp, Tyr and Phe. The 411 aa rhCPA2 consists of a pro region (aa 17 to 112) and |
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| a mature chain (aa 113 to 417). |
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| Catalog # | C1204-07 |
| Applications | Suitable for use in Western blot, Neutralization, Direct ELISA and Immunoprecipitation. Other applications have not been tested. |
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| Recommended Dilutions: |
| Western blot:1-2ug/ml the detection limit is~10 ng/lane under non-reducing and reducing conditions. will increase sensitivity by 5 to 50 fold. |
| Neutralization: preincubate with the activated enzyme at different molar ratios at room temperature for 30 min. Then assay with N-acetyl-Phe-Thiaphe-OH. 50% of the enzymatic activity was inhibited at~ 1.5 ug/ml (IC50) under conditions in which the enzyme was |
| present at 0.1 ug/ml and the substrate concentration was 0.1 mM. Considering the |
| molecular masses of the enzyme (42kD) and the antibody (150kD), IC50 was achieved at |
| approximately 5:1 molar ratio of the antibody to the enzyme. |
| Immunoprecipitation: 25 ug/ml |
| Direct ELISA: 0.5-1.0 ug/mL |
| Optimal dilutions to be determined bythe researcher. |
| Storage and Stability | |
| Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 8B4 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a lyophilized powder from PBS with 5% trehalose . Reconstitute with 1ml sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | NS0-derived recombinant human rhCPA2. |
| Specificity | Recognizes the pro form of human CPA2, but not the activated enzyme in Western blots. Species sequence homology: chimpanzee 99.5%, canine 90%, rat 87%, mouse 86% and chicken 70%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
