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You are here:Home » Antibodies » Abs to Enzymes, Carboxypeptidase » Anti -Carboxypeptidase A2

Anti -Carboxypeptidase A2

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
Carboxypeptidase A2 (CPA2) is a pancreatic peptidase that cleaves C-terminal residues with aromatic side chains including Trp, Tyr and Phe. The 411 aa human CPA2 consists of a pro region (aa 17 to 112) and a mature chain (aa 113 to 417).
Catalog #C1204-08
ApplicationsSuitable for use in Western blot, Immunoprecipitation, and Direct ELISA. Other applications have not been tested.
Recommended DilutionsWestern blot: 1-2ug/ml Using a colorimetric detection system, the detection limit for rhCPA2 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with WesternGloTM Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.
Immunoprecipitation: 25ug/ml
Direct ELISA: 0.5-1.0ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaNSO myeloma cells with spleen cells from Balb/c mice.
Endotoxin < 0.1 EU per 1ug of the antibody as determined by the LAL method.
Storage and Stability
Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No8B5
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived recombinant human rhCPA2.
SpecificityRecognizes human CPA2. Recognizes the pro form, (aa 17 - 417) but not the activated
enzyme (aa 113 - 417) in Western blots. Does not cross-react with rhCPA1, rhCPA4, rhCPB1 or
rhCPE. Species sequence homologyChimpanzee 99.5%,, canine 90%, rat, 87%, mouse 86% and chicken 70%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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