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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E B IH

Catalog #	C1209-06
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:2000-1:10,000; Assayed against 1ug of Carboxypeptidase B in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at RT.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile dH2O or 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Porcine
Concentration	~10mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% sodium azide. Reconstitute with 1ml deioinzed water or 40-50% sterile glycerol for long-term storage. Label
Purity	Purified from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.
Immunogen	Carboxypeptidase B (porcine pancreas)
Specificity	Recognized porcine Carboxypeptidase B. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, purified and partially purified carboxypeptidase B (porcine pancreas).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.