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You are here:Home » Antibodies » Abs to Enzymes, Carboxypeptidase » Anti -Carboxypeptidase Y

Anti -Carboxypeptidase Y

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IP
Catalog #C1211-05
ApplicationsSuitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoprecipitation and Conjugation. Other applications have not been tested.
Recommended DilutionELISA: 1:10,000-1:42,000
Western Blot: 1:2500. Both the antiserum and IgG fractions of anti-carboxypeptidase Y (Bakerís yeast) are shown to detect under reducing conditions of SDS-PAGE the 61kD enzyme in cellular extracts. Approximately 10ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP goat-a-rabbit IgG diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration10mg/ml
FormSupplied as a liquid from PBS, pH 7.2, 0.01% sodium azide
PurityPurified by delipidation, salt fractionation and ion exchange chromatography.
ImmunogenCarboxypeptidase Y (yeast).
SpecificityImmunoelectrophoretic assay gives a single precipitin arc against anti-rabbit serum, purified carboxypeptidase Y and partially purified carboxypeptidase Y (yeast). Although not tested, the antibody should exhibit crossreactivity with other tissues and species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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