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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E B IP Gel

Catalog #	C1211-05
Applications	Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoprecipitation and Conjugation. Other applications have not been tested.
Recommended Dilution	ELISA: 1:10,000-1:42,000
	Western Blot: 1:2500. Both the antiserum and IgG fractions of anti-carboxypeptidase Y (Baker’s yeast) are shown to detect under reducing conditions of SDS-PAGE the 61kD enzyme in cellular extracts. Approximately 10ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP goat-a-rabbit IgG diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	10mg/ml
Form	Supplied as a liquid from PBS, pH 7.2, 0.01% sodium azide
Purity	Purified by delipidation, salt fractionation and ion exchange chromatography.
Immunogen	Carboxypeptidase Y (yeast).
Specificity	Immunoelectrophoretic assay gives a single precipitin arc against anti-rabbit serum, purified carboxypeptidase Y and partially purified carboxypeptidase Y (yeast). Although not tested, the antibody should exhibit crossreactivity with other tissues and species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.