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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH

The carcinoembryonic antigen (CEA) is a member of a large family of glycoproteins and a useful tumor marker for adenocarcinoma. In the past, clinical utility of CEA antibodies in immunopathology has been limited by crossreactivity with CEA-related antigens, particularly Non-Speci c Cross-Reacting Antigen (NCA), in normal colon mucosa and human granulocytes.
Catalog #	C1299-87
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Positive Control Tissue	Colon carcinoma
Expected Staining Pattern	Cytoplasmic and membrane
Epitope Retrieval	Not Required
Enzyme Digestion	Not Required
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	3G57
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
Purity	Purified
Immunogen	Human colon carcinoma cell extracts
Specificity	Reacts speci cally with a restricted epitope on the carcinoembryonic antigen (CD66e, 180kD) in the gastrointestinal tract and does not react with normal colon tissue. Unlike most other CEA antibodies, does not react with Non-Speci c Cross-Reacting Antigen (NCA or CD66c, 55kD.) Col-1 is an established marker for gastric and colon adenocarcinoma. In a study of 35 different types of normal tissues, zero demonstrated positive immunoreactivity. Of 59 tested benign and malignant tumor tissues, reactivity was observed only in neoplasms of epithelial origin, particularly those from the gastrointestinal tract, breast, lung and bladder. Certain pre-malignant cells, such as colonic villous adenoma, also expressed this antibody.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.