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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -Carcinoembryonic Antigen (CEA)

Anti -Carcinoembryonic Antigen (CEA)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E IH
The carcinoembryonic antigen (CEA) is a member of a large family of glycoproteins and a useful tumor marker for adenocarcinoma. In the past, clinical utility of CEA antibodies in immunopathology has been limited by cross-reactivity with CEA-related antigens, particularly Non-Specific Cross-Reacting Antigen (NCA), in normal colon mucosa and human granulocytes.
Catalog #C1299-87O
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry Staining: Brightness 80, Cytoplasmic staining 3+, Membrane staining 2+, Background 2+, Staining of mucus 2+. Optimal dilutions to be determined by the researcher.
Recommended antibody pair for sandwich immunoassayCapture: C1299-87W
Detection: C1299-87O
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9B35
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM Tris, pH7.5, 0.15M sodium chloride, 0.05% sodium azide. Also available without azide. See C1299-87S.
Purity~90% by SDS-PAGE. Purified by ion-exchange chromatography.
ImmunogenHuman natural tumor-derived purified CEA antigen
SpecificityRecognizes human carcinomas. Epitope specificity group IVa or II. Does not crossreact with human leukocytes in cytofluorometry.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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