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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E IH

Carcinoembryonic Antigen (CEA) is synthesized during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
Catalog #	C1300-01B
Applications	Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: Can be used for detection in sandwich ELISA with C1300-01C (coating).
	Immunohistochemistry (Paraffin): Antigen retrieval is not essential but may optimize staining.
	Optimal dilutions to be determined by the researcher.
Hybridoma	Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8J503
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in 10mM Tris, 0.15M sodium chloride, 0.05% sodium azide.
Purity	Purified
Immunogen	Human natural tumor-derived purified carcinoembryonic antigen. Cellular Localization
Specificity	Recognizes human Carcinoembryonic Antigen (CEA). Does not crossreact with human leukocytes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.